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| Zhou, Jinlin; Yang, Jun; Zhang, Guohong; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan. |
| A cDNA encoding the apical membrane antigen-1 (AMA-1) homologue was obtained by immunoscreening a cDNA expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 2062 bp. Computer analysis suggested that the sequence contains an open reading frame of 1794 bp with a coding capacity of approximately 66 kDa. Based on the homology analysis, this putative protein was designated as B. gibsoni AMA-1 (BgAMA-1). The BgAMA-1 gene was expressed in the Escherichia coli BL21 strain and used as the antigen in Western blotting and the enzyme-linked immunosorbent assay (ELISA). The results indicated that BgAMA-1 was recognized as an immunodominant antigen by the host immune system and that it induced a strong antibody... |
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Palavras-chave: Babesia gibsoni; AMA-1; Antibody response. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/815 |
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